Biobank infrastructure


The TBB infrastructure is placed within the Institute of Pathology. Every sample received for Biobanking is divided for snap frozen and RNAlater® fixation in a barcoded tube system from FluidX® linked to the database for an optimal tracking and automated storage location assignment of the sample. This 1D – 2D double barcoded polypropylene tubes are sterilized and free from DNases, RNases, nucleic acids, and endotoxins.

 

 

To assure the quality of the tissue at arrival ischemia times are included in the patient report so they can be tracked from the operation room. The sample transportation from the operation room is automated and done in less than 2 minutes in most cases.

 

The corresponding normal tissues for every patient are handled and stored similarly; FFPE material is also available for tumor and normal samples.

 

We also offer the possibility to give researchers fresh material at the time of the tissue reception to establish primary cell lines. At the moment there are 8 different prospective projects for primary cell line establishment successfully ongoing encompassing 6 different tissue types.

 

Storage

Right after diagnosis, samples are flash frozen to -70 °C in a pre-cooled Isopentane tank and transferred to a -80°C freezer for long-term storage. All the TBB freezers are equipped with the Central Monitoring System ELPRO® that takes and stores temperature data every 5 minutes and sends an alarm at a fixed temperature. This ensures the correct temperature maintenance and tracking for an optimal tissue quality preservation.